Verification away from recombination situations from the Sanger sequencing
In using 2nd-generation sequencing, detection away from non-allelic succession alignments, in fact it is due to CNV or unfamiliar translocations, is actually worth addressing, as the failure to determine them can result in incorrect positives getting both CO and you may gene conversion process incidents .
From this filtering, a total of approximately 20% small double CO or gene transformation individuals was basically omitted on account of the fresh openings from the source genome or not clear allelic dating
To understand multi-backup regions i utilized the hetSNPs named when you look at the drones. Commercially, the brand new heterozygous SNPs will be simply be noticeable from the genomes regarding diploid queens however throughout the genomes out of haploid drones. But not, hetSNPs are also titled when you look at the drones on up to twenty-two% out-of king hetSNP websites (Dining table S2 in the Extra document dos). To own 80% of those websites, hetSNPs have been called in at least one or two drones and have linked in the genome (Table S3 within the More document 2). On top of that, notably large see visibility are understood on drones within these websites (Shape S17 inside the A lot more document 1). An educated need for these hetSNPs is they are the consequence of content count variations in the fresh new selected territories.
